ki67 antibody 6  (Bioss)

Journal: Human Cell

Article Title: Inhibitory effects of the combination of rapamycin with gemcitabine plus paclitaxel on the growth of pancreatic cancer tumors

doi: 10.1007/s13577-024-01165-9

Figure Lengend Snippet: Effects of rapamycin on apoptosis and the inhibition of cell proliferation in vitro. A Western blotting was performed on S2-013 and PANC-1 cells treated with rapamycin using an anti-phosphorylated p70S6K antibody. B , C The MTT assay was performed on S2-013 ( B ) and PANC-1 ( C ) cells treated with rapamycin, gemcitabine, GP, rapamycin plus GP, 5-FU, and rapamycin plus 5-FU. The concentrations of rapamycin were 20 and 100 nM, that of gemcitabine was 10 nM, that of paclitaxel was 10 nM, and that of 5-FU was 40 μM. Data were derived from three independent experiments. Columns , mean; bars , SD. * p < 0.05 significantly different from non-treated control cells (the Student’s t -test). D Confocal immunofluorescence microscopic images of Ki-67 (green) in S2-013 cells treated with rapamycin, GP, and rapamycin plus GP. Blue, DAPI staining. Scale bars, 10 μm. Quantification of data; values represent the number of cells stained with the anti-Ki-67 antibody. All cells in four visual fields per group were scored. Data were derived from three independent experiments. Columns, mean; bars, SD. * p < 0.05 significantly different from non-treated control cells (Fisher’s exact test). E Western blotting was performed on S2-013 cells treated with rapamycin, GP, and rapamycin plus GP using anti-cleaved caspase-3 and anti-cleaved caspase-7 antibodies (left panel). Rapamycin was used at a concentration of 100 nM, while gemcitabine and paclitaxel were both used at 10 nM. When combined, all drugs, except rapamycin, were used at the same concentration (10 nM), with rapamycin being used at a lower concentration of 20 nM. A baseline value was calculated by subtracting the blank value from the measured GAPDH value in the group (control, rapamycin, GP, and rapamycin plus GP) (right panel). The value for each antibody (anti-cleaved caspase-3 and anti-cleaved caspase-7 antibodies) was then calculated by subtracting the blank value from the corresponding measured value. The ratio to the baseline was assessed by dividing the antibody value by the baseline value for the group. The combination of rapamycin with GP increased the ratio of cleaved caspase-3 and −7, indicating that this combination induced apoptosis

Article Snippet: An anti-Ki-67/MKI67 (SP-6) antibody (NB600-1252) was purchased from Novus Biologicals (Centennial, CO).

Techniques: Inhibition, In Vitro, Western Blot, MTT Assay, Derivative Assay, Control, Immunofluorescence, Staining, Concentration Assay

Journal: Human Cell

Article Title: Inhibitory effects of the combination of rapamycin with gemcitabine plus paclitaxel on the growth of pancreatic cancer tumors

doi: 10.1007/s13577-024-01165-9

Figure Lengend Snippet: Post-treatment effects of rapamycin and everolimus on tumor cell proliferation and apoptosis. A Immunohistochemical staining with the anti-Ki-67 antibody of xenografts obtained from S2-013-organoid mice after the completion of treatment with rapamycin, GP, and rapamycin plus GP. Bars, 50 µm. B TUNEL assay on xenografts obtained from S2-013-organoid mice after the completion of treatment with rapamycin, GP, and rapamycin plus GP. Values represent the number of TUNEL-positive cells. All cells in four fields per group were scored. Data were derived from three independent experiments. Columns, mean; bars, SEM. * p < 0.05 significantly different from non-treated control cells (the Student’s t -test). Bars, 50 µm. C Immunohistochemical staining with the anti-Ki-67 antibody in xenografts obtained from S2-013-organoid mice after the completion of treatment with everolimus, GP, and everolimus plus GP. Bars, 50 µm. D TUNEL assay on xenografts obtained from S2-013-organoid mice after the completion of treatment with everolimus, GP, and everolimus plus GP. Values represent the number of TUNEL-positive cells. All cells in four fields per group were scored. Data were derived from three independent experiments. Columns, mean; bars, SEM. Bars, 50 µm

Article Snippet: An anti-Ki-67/MKI67 (SP-6) antibody (NB600-1252) was purchased from Novus Biologicals (Centennial, CO).

Techniques: Immunohistochemical staining, Staining, TUNEL Assay, Derivative Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Bisphenol-A in Drinking Water Accelerates Mammary Cancerogenesis and Favors an Immunosuppressive Tumor Microenvironment in BALB– neu T Mice

doi: 10.3390/ijms25116259

Figure Lengend Snippet: Expression of proliferation, neoangiogenesis and apoptosis markers in mammary tumor tissues from BALB– neu T mice. Mammary tissues were collected from BPA-treated and untreated (CTR) mice at the preinvasive or invasive stage of tumor progression (n = 3) and immunostained for markers of ( A ) proliferation (Ki67), ( B ) neoangiogenesis (CD31), and ( C ) apoptosis (cleaved caspase 3). The immunostaining was scored as described in . Tissue sections were counterstained with hematoxylin. The results are expressed as the mean ± SD values of three independent experiments performed in triplicate (** p ≤ 0.01; *** p ≤ 0.001 vs. CTR). Arrows indicate CD31-positive vessels. Images were acquired with an OLYMPUS BX53 microscope (original magnification 200×).

Article Snippet: The anti-F4/80 (CI:A3-1; cat. no. #BE0206; 1:100) antibody was obtained from BioXcell (Lebanon, NH, USA) and the antibody against Ki67 (SP-6; cat. no. #MA5-14520; 1:250) from Invitrogen (Milan, Italy).

Techniques: Expressing, Immunostaining, Microscopy